The Use of the Green Fluorescent Protein and Its Application in the Study of Biochemistry

Green Fluorescent ProteinApplications in Biochemistry, Cell Biology and BiotechnologyThe squirt hoy protein (GFP) of Aequorea victoria is a unique in vivo sense organ for supervise dynamic processes in electric cells or organisms (Gerdes & adenosine monophosphate; Kaether 1996, p.44). This northerly Atlantic organism is highly organized in structure, with an awed great power to componentrate and emit green fluorescent light from photocytes at the margin of its umbrella, qualification it a perfect work for cellular studies. The molecular cloning of GFP and its demonstration by Chalfie (1988) has open up exciting new avenues of investigation in cell biology. The broad flexibility as a non-invasive scoring in quick cells allows for numerous applications of GFP (Yang F. et al. 1996, p.1246) such as a reporter for gene expression, as well as a marker to strike cell lineage during development. Thus, its ability to function as a protein tag allows us to localise proteins in l ive cells, as it tolerates N- or C- terminal fusions to a broad mannikin of proteins (Tsien R.S. 1998, p.511). These applications of GFP ar now found to be more weapons-grade to naturalized methods in cell biology. GFP is a 238 amino unpleasant protein with an unembellished molecular weight of about 27-30 kDa on SDS-PAGE (Gerdes & Kaether 1996, p.44).

The precious cylindrical fold of the protein seems ideally suited for its function, where ii promoters fellow to bumher to form a dimer in the crystal, making it fitting to undergo energy transfer (Yang F. et al. 1996, p.1248). Eleven anti-parallel genus Beta strands use up up the densely compact beta-barrel,! where slender sections of the alpha whorl form internal caps on the cylinders (Prasher D.C. et al. 1992, p. 229). This temporary alpha spiraling segment provides a scaffold for the chromophore which is regain in the nonrepresentational centre of the cylinder. The structure is... If you want to get a luxuriant essay, order it on our website: OrderCustomPaper.com

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