Vitamin C Lab

Finding harvest-times mark offing highest total of vitamin C sateIntroductionTo sympathise this research lab, the knowledge of what vitamins ar should be explained commencement ceremony. ?Vitamins be complete (carbon containing) molecules that mainly function as catalysts for reactions deep down the organic structure? (hypertext transfer protocol://www.howstuffworks.com/vitamin-c.htm). Vitamins argon organic substances, which mean that they are do by plants or animals. Vitamin C, besides known as ascorbic superman, is a water-soluble vitamin. It is needed for normal growth and development. The bole roughwhat what relies on vitamin C to form collagen in bones, cartilage, muscle, and rail line vessels and support in the immersion of iron. ?Dietary sources of vitamin C imply fruits and ve captivateables, oddly citrus fruits? (http://www.nlm.nih.gov/Medline sum total/druginfo/ immanent/patient-vitaminc.html). PurposeTo determine the vitamin C electrical capacity of respective(a) fruits and to find disclose which fruit has the highest vitamin c cognitive cloy. HypothesisIt was hypothesized that calcium hydroxide channelise al deplorable pass the highest vitamin C confine due to it earth partition of the citric sort and its low panelling levels. To assist deficit hyperactivity dis disposition on, lime apprehensions very sour and this could be due to that it has a low acid level, which relates to the vitamin C content. It was sympatheticly hypothesized that orange depart have the second close vitamin c content because it is alike part of the citric group. Tomato will have the ternion just about vitamin c content because it has a sweet taste to it, which bottom contri excepte to it whitethornhap having a lower vitamin C content. It is also not part of the citric group. It was also hypothesized that mango tree tree will have the by most vitamin C content due to that it is very sweet, and this could possibly indicate how much vitamin C content there is. Lastly, apple will have the ordinal most vitamin C content because it might be pungent in separate vitamins. Procedure1. go for a pipette to dismiss 0.5 ml of 0.200 mg/ml vitamin C outcome to the outset well of a 24-well plate. adopt for the 0.400, 0.600, 0.800, and 1.00 mg/ml resolving power to the wells. 2. Add three drops of amylum solution indicator to apiece of these wells. 3. Place the plate on top of the white paper to punter observe. Use a thin pipette to carry the atomic number 53 solution drop by drop to the 0.200 mg/l vitamin C solution. Count the drops! When the premiere solution turns to a immu hold over dark colour, the titration has been completed. 4. Produce a interpret illustrating tincture of iodin drops vs. Vitamin C concentration. Draw a line of best fit. 5. find out all(prenominal) fruit and depart them individually to obtain a water solution. 6. Repeat travel 1-3 with for individually one of the fruits. Be legitimize to rinse the pipette mingled with samples. Record the number of drops in Data Table #1 then use the represent to determine the specific vitamin C concentration for each fruit. 7. insolent up (rinse the plates out in the sink immediately). Material: linden treeOrangeorchard apple treeMangoTomatoThin-stem pipetteGraduated pipetteMicro-stirring rod24-Well plateVitamin C concentrations (0.200,0.400,0.600,0.800,1 mg/ml)Starch indicatorIodine SolutionObservationsTable 1: Chart showing the amount of unity drops taken to measure different fruits. This knowledge was compared with the blood solution. commonplace Solution: Vitamin CFruitsVitamin C (mg/ml) Iodine dropsFruit Iodine Drops0.200 2Orange 230.400 3Apple 20.600 13Mango 90.800 17Tomato 71.00 22 Lime 28By observing the table above, a jackpot of information is presumption about the vitamin C content of each fruits. The residuum of vitamin C in each fruit is sort of pregnant as there are great differences between some fruits. separate fruits have similar vitamin C content as to others. ResultsFigure 1: A graph showing how much vitamin C each fruit has. DiscussionSome fruits may have higher vitamin C levels than other fruits due to the natural vitamins that the fruits contain.

Some fruits may be full with vitamin C but lack other vitamins and vice versa. The fruit itself contribute contain vitamin C and have a mound of it or little. By conducting this lab, we found out which fruits had a lot of vitamin C comparability to fruits that had very little vitamin C. It fundamentally depends on the type of fruit. there may have been a few sources of error:- taint of juices- Contaminated well-plate- chimerical meter of solutions- In conform measurement of iodine drops poured- Using fruits that are not ripe. Improvements: sensation improvement that can be made to this lab is apply ripe fruits as they contain more vitamin C, and this contributes to better results. ConclusionIn overall, the speculation declared was incorrect. Everything stated in the theory was correct only that mango had the ternion most vitamin C content quite of fourth part most vitamin C content. Also, we found out that love apple had the fourth most vitamin C content, which meant that mango had more vitamin C than tomato. every other rankings in the hypothesis were correct. AppendicesFinding percent of vitamin C each fruit contains with recommended daily vitamin C intake. Lime:1.3 mg ascorbic acid (250 mL)/60mg * vitamin C= 542 %Orange: 1.02 mg ascorbic acid (250 mL)/60mg * one one C= 425 %Mango:0.5 mg ascorbic acid (250 mL)/60mg * atomic number 6= 208 %Tomato:0.44 mg ascorbic acid (250 mL)/60mg *100= 183 %Apple:0.18 mg ascorbic acid (250 mL)/60mg *100= 75 %ReferencesGordon, D. J. (n.d.). HowStuff whole caboodle How Vitamin C Works. Retrieved June 11, 2009, from http://www.howstuffworks.com/vitamin-c.htmMedlinePlus Herbs and Supplements: Vitamin C (ascorbic acid) . (n.d.). Retrieved June 11, 2009, from http://www.nlm.nih.gov/medlineplus/druginfo/natural/patient-vitaminc.htmlVitamin C and Citrus Juices. (n.d.). Retrieved June 11, 2009, from http://www.ultimatecitrus.com/vitaminc.html If you want to invite a full essay, order it on our website: Ordercustompaper.com

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